As one of two principal investigators for the Holt/Géléoc Lab at Boston Children's Hospital, Dr. Holt is investigating the function of ion channels genes in hair cells using viral vectors to deliver wild-type and mutant channel genes. He is also interested in identifying the genes and proteins required for hair cell mechanosensory transduction and the discovery of viral vectors to restore cellular and systems level function in models of human deafness.

He earned his doctorate from the Department of Physiology at the University of Rochester. For his thesis, he studied inward rectifier potassium currents in saccular hair cells in Dr. Ruth Anne Eatock's lab. He then completed a post-doc with Dr. David Corey in the Neurobiology Department at Harvard Medical School.