University Hospital Southampton NHS Foundation Trust is an NHS foundation trust which operates the Southampton General Hospital, the Princess Anne Hospital, Southampton Children’s Hospital, and the New Forest Birth Centre at Ashurst, Hampshire. It also provides a few services at the Royal South Hants Hospital and previously operated Countess Mountbatten House, a palliative care service at Moorgreen Hospital, which has since been renamed Mountbatten Hampshire.

The trust has one of the 11 Genomics Medicines Centres associated with Genomics England which opened across England in February 2014. It is one of the biggest provider of specialised services in England, which generated an income of £262.2 million in 2014/5. In September 2016, the trust was selected by NHS England as one of twelve Global Digital Exemplars.