Since the launch of "The R-Files" in February 2024, a series focused on all aspects of rapamycin, numerous questions have poured in about this compound and its role in ageing and longevity. Every comment from platforms like YouTube, Twitter, and LinkedIn has been reviewed to compile a list of inquiries about combining interventions, optimizing rapamycin dosing, the limitations of applying findings from mouse models and clinical studies to off-label human use of rapamycin and other supplements, and much more.

Key Points:

This episode of "The R-Files" is focused on answering audience questions about rapamycin, particularly in the context of ageing and longevity. It addresses topics such as combining interventions with rapamycin, optimizing its dosing, and exploring the challenges of applying research findings. 

• Caloric Restriction vs. Rapamycin: The episode explores whether rapamycin mimics caloric restriction. While both target similar ageing pathways, they function differently, and the potential benefits of combining them remain unclear in humans.
• Cancer Mortality and Rapamycin: Rapamycin delays cancer progression in mice but does not prevent it. Caloric restriction shows stronger anti-cancer effects, though both interventions broadly slow ageing.
• Life Extension Techniques: The podcast discusses combining different longevity interventions like rapamycin and growth hormone signalling. However, research on the additive benefits of such combinations is limited.
• Metformin and Rapamycin: Combining metformin with rapamycin is popular due to potential overlapping benefits, but evidence of an additive effect is still speculative.
• Rapamycin Dosing and Efficacy: Dosing is highly individualized, and testing rapamycin blood levels may help optimize dosing. The episode emphasizes the variability in how rapamycin is metabolized.