Key points from article :
CRISPR-Cas9, modified, now less dependent on protospacer adjacent motif (PAM).
Overcoming that limitation, allowing them to target any location on entire genome.
Team created variants of Cas9 protein not requiring specific PAM to bind to and cut DNA.
Two variants created, SpG and SpRY, more efficient than regular Cas9 enzyme.
New approach allows targeting of previously inaccessible regions of the genome.
Next step, develop deeper understanding of mechanisms involved in Cas9 protein variants.
Researchers from Massachusetts General Hospital, published in Science.
