Research Expertise and Interest

RNA machines, hepatitis C virus, RNA interference, ribosomes, x-ray crystallography, cryo-electron microscopy, CRISPR-Cas9 genome editing

Research Description

RNA forms a variety of complex globular structures, some of which function like enzymes or form functional complexes with proteins. Our research into RNA biology led to the discovery of CRISPR-Cas9 as a tool for making targeted changes to the genome. In bacteria, CRISPR systems preserve invading genetic material and incorporate it into surveillance complexes to achieve adaptive immunity. Crystal structures of diverse Cas9 proteins reveal RNA-mediated conformational activation. Current research in the Doudna lab focuses on discovering and determining the mechanisms of novel CRISPR-Cas and associated proteins; developing genome editing tools for use in vitro, in plants, and in mammals; and developing anti-CRISPR agents.