Key points from article :
A new scaffold for liver regeneration has been characterised.
Harnessing liver's regenerative capacity in vitro is difficult.
Hepatocytes are sensitive to their environment and lose their phenotype once outside.
A microenvironment for hepatocytes to preserve their liver-specific behaviour has been created.
Potential of fibrin incorporated hydrogel for hepatic tissue engineering was explored.
A three-hydrogel system was created containing alginate, gelatin and fibrin(ADA-G-FIB).
A hepatocyte cell line was cultured in the hydrogel.
Morphology, viability and functionality analysis showed excellent results.
Cells grown on the fibrin combination hydrogel outperformed 2D culture.
ADA-G-FIB hydrogel may be used as an effective 3D system for liver tissue engineering applications.
Research by Sree Chitra Tirunal Institute for Medical Sciences and Technology published in International Journal of Biological Macromolecules.
